Fig. 10

PLX5622 treatment corrects dendritic spine density and morphology in the somatosensory cortex (A–D), hippocampal CA1 (E–H), and striatum (I–L) of the VPA-model mice. A, E, I, Representative images of dendritic spines in the somatosensory cortex (A), hippocampal CA1 (E), and striatum neurons (I) of four groups of mice: “Saline P21” (the Saline group of mice at P21), “VPA P21” (the VPA group of mice at P21), “Saline P52” (the Saline group of mice at P52) and “VPA rep. 21” (the PLX5622-treated VPA group of mice at P52). Scale bar: 7 μm (A) or 10 μm (E and I); n = 3 for each group. B, F, J, Representative 3D image of dendritic spines reconstructed by Imaris 9.9 for (A), (E) and (I), respectively. C, G, K Total number of dendritic spines contained in 10 μm dendrites. n = 6–9 per group; one-way ANOVA. D, H, L Statistical analysis of the number of mushroom and stubby dendritic spines contained in 10 μm dendrites. n = 6–9 per group; two-way ANOVA. ***p < 0.001, **p < 0.01, *p < 0.05. Data are presented as mean ± SEM