Fig. 6

Clec5a knockout increases the plaque-associated microglia in the AD mouse model. (A) The methodology for assessing the coverage of plaque-associated microglia labeled with Iba1 (green) and Aβ (red) antibodies. DAPI (blue) was used to indicate the nuclei. The yellow circle represents the region covering the entire Aβ plaque. The red circle, expanded by a 10 μm radius from the yellow circle, represents the plaque-associated area. Scale bar: 20 μm. (B) The plaque-associated microglia coverage was calculated as the ratio of the microglia area in the yellow circle to the plaque area in the red circle. (C) The correlation between plaque-associated microglia coverage and plaque size was determined using Pearson correlation analysis. Dashed line: 95% confidence interval bands. Solid line: best-fit line. n = 88 plaques for APP, n = 110 plaques for APP5A^−/− from 5 mice in each genotype. (D) The representative images of CD68 (red) and Iba-1 (green) double staining. DAPI (blue) was used to indicate the nuclei. Scale bar: 30 μm. (E) The microglial CD68 area was calculated and normalized to the WT group. n = 8–14 slices for each mouse. 8 mice for the APP group and 6 mice for the APP5A^−/− group were analyzed. *p ≤ 0.05