Fig. 5
Clec5a knockout reduces the Aβ accumulation in the AD mouse model. (A, B) The levels of guanidine soluble total Aβ (A) and the Aβ1−42 (B) in mouse hippocampus were measured by ELISA. n = 12 for APP, n = 22 for APP5A−/−. (C-F) Amyloid plaques were analyzed using Thioflavin-S (Thio-S) and anti-Aβ antibody staining. Representative images of Thioflavin-S+ staining (C) and Aβ antibody staining (D). The number of Thioflavin-S+ plaques (E) and the percent area covered by plaques (F) in the hippocampus. 3–7 slices per mouse and 5–7 mice in each genotype were analyzed. Scale bar: 200 μm. (G) The size of each plaque was determined from the Aβ antibody staining. n = 88 plaques for APP, n = 110 plaques for APP5A−/− from 5 mice in each genotype. *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001