Fig. 6

The mechanism of MCH in neuroinflammation induced by IAV infection. (A-B) Expression of MKP-1, p-JNK, JNK, p-ERK, and ERK proteins detected by western blot and the quantitative analysis, n = 6. (C) Sleep latency and sleep duration, n = 12. (D) The swimming velocity, escape latency, path length, and time spent in the target quadrant in the Morris water maze test, n = 12. (E) The movement trail map of mice in the Morris water maze test, n = 12. (F) The levels of IL-1β and TNF-α detected by ELISA, n = 6. (G) Representative Nissl staining in the hippocampal DG and CA1 regions and cortex. Bar = 100 μm, n = 6, 5 slices for each mouse. (H) Representative immunofluorescence staining of Iba-l⁺ cells in the hippocampus and cortex. Bar = 50 μm, MCH-H, high dose MCH, n = 6, 5 slices for each mouse. Data were expressed as mean ± SEM, **P < 0.01, ***P < 0.001