Fig. 5

Effect of MCH on the level of inflammatory cytokines and JNK/ERK signaling pathway in influenza-induced mice. (A-B) The levels of IL-1β and TNF-α detected by ELISA, n = 6. (C) Expression of MKP-1, p-JNK, JNK, p-ERK, and ERK proteins in the hippocampous detected by Western blot. (D-F) Quantitative analysis of the ratio of p-JNK/JNK and p-ERK/ERK. Data were expressed as mean ± SEM, n = 6 for ELISA assay and Western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001