Table 3 Multivariate linear regression analysis of microglial basal gene expression. Data show intercept (control animal receiving saline in postnatal period), β (SE) and p-values for sex and time of postnatal LPS exposure for each gene. Ns, not significant. Male basal gene expression (sal/sal) was set to 1 for each gene and gene expression in females was calculated relative to males
Cortex | IL6 | iNOS | Arg-1 | Ptgs2 | C/EBP | Cxcl10 | CD14 | CD68 | Tmem119 | P2Y12 |
|---|---|---|---|---|---|---|---|---|---|---|
intercept | 1.01 (0.08) | 1.15 (0.11) | 1.26 (0.19) | 1.03 (0.08) | 1.06 (0.08) | 1.37 (0.48) | 1.07 (0.07) | 1.04 (0.06) | 1.06 (0.06) | 1.07 (0.08) |
Sex (F) | 0.05 (0.10) ns | -0.00 (0.13) ns | -0.28 (0.22) ns | 0.04 (0.10) ns | -0.00 (0.09) ns | -0.70 (0.58) ns | -0.01 (0.08) ns | 0.00 (0.08) ns | -0.05 (0.07) ns | 0.00 (0.10) ns |
P7 | -0.18 (0.13) ns | -0.36 (0.18) ns | 0.27 (0.31) ns | 0.00 (0.13) ns | -0.22 (0.13) ns | 2.07 (0.80) p < 0.0120 | -0.16 (0.11) ns | 0.04 (0.10) ns | 0.01 (0.10) ns | -0.03 (0.14) ns |
P12 | 0.10 (0.14) ns | -0.16 (0.19) ns | 0.70 (0.31) P < 0.0303 | 0.03 (0.14) ns | 0.23 (0.14) ns | 0.15 (0.83) ns | 0.08 (0.11) ns | 0.22 (0.11) P < 0.0488 | 0.12 (0.10) ns | 0.05 (0.146 ns |
P18 | -0.33 (0.14) P < 0.0218 | -0.18 (0.18) ns | -0.00 (0.32) ns | -0.00 (0.14) ns | 0.02 (0.13) ns | 0.13 (0.82) ns | -0.19 (0.11) ns | -0.05 (0.11) ns | -0.07 (0.10) ns | 0.02 (0.14) ns |
Cervical Spinal Cord | IL6 | iNOS | Arg-1 | Ptgs2 | C/EBP | Cxcl10 | CD14 | CD68 | Tmem119 | P2Y12 |
intercept | 1.06 (0.08) | 1.22 (0.13) | 1.08 (0.24) | 1.14 (0.09) | 1.18 (0.13) | 1.03 (0.07) | 1.11 (0.10) | 1.02 (0.06) | 1.17 (0.12) | 1.00 (0.06) |
Sex (F) | 0.04 (0.09) ns | -0.05 (0.16) ns | 0.12 (0.29) ns | -0.06 (0.11) ns | -0.03 (0.16) ns | 0.06 (0.08) ns | -0.01 (0.12) ns | 0.02 (0.08) ns | -0.04 (0.14) ns | -0.01 (0.07) ns |
P7 | -0.36 (0.14) p < 0.0122 | -0.12 (0.23) ns | -0.04 (0.41) ns | -0.12 (0.15) ns | -0.36 (0.23) ns | 0.10 (0.12) ns | -0.15 (0.18) ns | 0.18 (0.11) ns | 0.06 (0.20) ns | -0.06 (0.10) ns |
P12 | -0.19 (0.14) ns | -0.47 (0.23) P < 0.0506 | 0.01 (0.43) ns | -0.32 (0.16) p < 0.0518 | -0.22 (0.23) ns | 0.03 (0.12) ns | -0.03 (0.18) ns | -0.02 (0.12) ns | -0.10 (0.21) ns | -0.02 (0.11) ns |
P18 | -0.22 (0.13) ns | -0.41 (0.21) ns | 0.50 (0.40) ns | -0.14 (0.1) ns | 0.08 (0.21) ns | 0.09 (0.11) ns | 0.04 (0.17) ns | 0.03 (0.11) ns | 0.18 (0.19) ns | 0.01 (0.10) ns |