Fig. 3

Central IL-2 administration improved insulin sensitivity compared to pair-fed mice. A. Illustration of central IL-2 administration. B. Cumulative food intakes between the vehicle-administered pair-fed group and the IL-2 administered-group (n = 5 for pair-fed group and n = 6 for IL-2 group). C. Graphs of body weights and body weight changes between the vehicle-administered pair-fed group and the IL-2 administered group (n = 5 for pair-fed group and n = 6 for IL-2 group). D. Representative images and quantification of H&E staining of BAT and iWAT of the pair-fed or IL-2-administered mice (n = 5 for pair-fed group and n = 6 for IL-2 group). Scale bars: 50 μm. E. Insulin tolerance test was performed on the 8th day after starting pair-feeding (n = 5 for pair-fed group and n = 6 for IL-2 group). F and G. Flow cytometry analysis of Th1 cells (CD45 + TCRβ + CD4 + IFNγ+) and Treg cells (CD45 + TCRβ + CD4 + CD25 + FoxP3+) in gWATs (n = 5 for pair-fed group and n = 6 for IL-2 group). Results are presented as mean ± SEM. Statistical analyses were performed using one-sided two-way ANOVA (B, C [left], D [lower], E [left]) and two-sided Student’s t-test (C [right], D [upper], E [right], F, G). *p < 0.05 and ***p < 0.001 between the indicated groups. NS, not significant