Fig. 1

Systemic IL-2 administration improved insulin sensitivity in HFD-induced obese mice. A. Illustration of systemic IL-2 administration and the experimental timeline. B and C. Changes in body weight and food intake during intraperitoneal injection of vehicle or IL-2. The arrows indicate the time points of metabolic analysis (n = 5). D and E. Glucose and insulin tolerance tests were performed on the 5th and 9th day after the first vehicle or IL-2 administration (n = 5) F and G. Flow cytometry analysis of Th1 cells (CD45 + TCRβ + CD4 + IFNγ+) and Treg cells (CD45 + TCRβ + CD4 + CD25 + FoxP3+) in gWATs of vehicle and IL-2 [1,000 IU] groups (n = 5) H. Comparison of mRNA expressions of inflammation-related genes, including Il-1β, Il-6, Il-8, Tnfα, Il-4, Il-10, and Tgfβ (n = 5) Results are presented as mean ± SEM. Statistical analyses were performed using one-sided two-way ANOVA (B, C, D [left], E [left]), one-sided one-way ANOVA (D [right], E [right]), and two-sided Student’s t-test (F, G, H). *p < 0.05, **p < 0.01, and ***p < 0.001 between the indicated groups. NS, not significant