Fig. 2

Specific co-expression of CX3CR1 and UCHL1 in microglia. (A) Quantification of the relative mRNA expressions of Cx3cr1 and Uchl1 in primary cultured Bone marrow-derived macrophage (BMDM) and microglia by qPCR. (B) Representative images of WB showed the expression of CX3CR1 and UCHL1 in primary cultured BMDM and microglia. (C) Representative immunostaining images for CX3CR1 and UCHL1 in primary cultured BMDM and microglia. (D) Images for double labeling CX3CR1 and UCHL1 in the EVs from BMDM and microglia and quantification of the double positive particles by Cytoflex LX. (E) Representative immunostaining images for CX3CR1 and UCHL1 in human brain, liver, stomach, and esophagus sections. (F) Representative immunostaining images for CX3CR1 and UCHL1 colocalization in different cell types (IBA1 for microglia, NEUN for neuron, GFAP for astrocyte, CC1 for oligodendrocyte, CD31 for brain microvascular endothelial cells) in human brain sections. All values are mean ± SEM. n = 3–4 in each group. ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001