Fig. 1

UCHL1 was a highly specific protein in BV2 EVs confirmed by mass spectrum screening. (A) Characteristic electron microscopy images of the EVs derived from BV2 and RAW264.7. (B) Representative NTA images showed the population of EVs derived from BV2 and RAW264.7. (C) WB images showed the exosome markers (TSG101, CD63, and ALIX) expressed in the EVs of BV2 and RAW264.7. (D) The bar plot and Volcano plot showed the different proteins between BV2 and RAW264.7 EVs. (E) KEGG analysis of different proteins between BV2 and RAW264.7 EVs. (F) GO analysis of different proteins between BV2 and RAW264.7 EVs. (G) qPCR verified the top 10 of different proteins between BV2 and RAW264.7. (H) WB images showed the UCHL1 expression in BV2 and RAW264.7 and the EVs secreted from those cells. (I) Quantification of the relative integrated intensity of cell protein and EVs protein in BV2 and RAW264.7 by WB. (J) Typical WB images for CX3CR1 and UCHL1 and their quantification of relative integrated intensity in different mouse organs. ^***p < 0.001 versus other groups. (K) Representative images for double labeling CX3CR1 and UCHL1, along with the quantification of double-positive particles in BV2 and RAW264.7 EVs. All values are mean ± SEM. n = 3–4 in each group. ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, ns, no significance