Fig. 2

Behavioral tests on motor and cognitive function of LPC mice with SIRT6 inhibitor treatment A Schematics of treatment strategies. The SIRT6 selective inhibitor OSS_128167 was bilaterally injected into the ventricle after LPC injection into CC, and behavioral tests were conducted between 14 ~ 28 dpi. Tissues were collected for histopathological, biochemical analyses at 7, 14, 28 dpi. B Olig2, PDGFRα, and CNPase mRNA levels were measured at 14 dpi in demyelinated lesions by qPCR (one-way ANOVA with Tukey’s multiple-comparison test, n = 3 per group). C Escape latencies of the four groups of mice during training days of the Morris water maze (MWM) (two-way ANOVA). D Time of mice spent in the target quadrant in the probe trial of the MWM. E Number of platform crossings during the probe test in the MWM task. F Representative swim trajectories of each group in orientation navigation trials and spatial exploration trials. G Preference index of the novel object. H, I Evaluation of motor function using OFT and Rotarod test in four groups of mice. Statistical analysis was performed using one-way ANOVA and followed by Tukey’s multiple comparisons test, n = 6 per group. Data: P < 0.05 is statistically significant. All values are denoted as the mean ± SD