Fig. 6

Htr7⁺ Tregs ameliorated LPS-induced neuroinflammation in Rag1^−/− mice. A Representative dot plots of CD206⁺ microglia in the hippocampus of Rag1^−/− mice adoptively transferred to Tregs transfecting with the lentivirus Htr7-Flag (n = 7/group). B Percent of flow cytometry data for CD206⁺ microglia in the hippocampus of Rag1^−/− mice (n = 7/group). C Absolute number of MHC-II⁺ microglia in the hippocampus of Rag1^−/− mice (n = 7/group). D Percent of CD206⁺ macrophage in the hippocampus of Rag1^−/− mice determined by flow cytometry (n = 7/group). E Absolute number of MHC-II⁺ macrophage in the hippocampus of Rag1^−/− mice (n = 7/group). F Absolute number of MHC-I⁺ microglia in the hippocampus of Rag1^−/− mice (n = 7/group). G Absolute number of MHC-I⁺ macrophage in the hippocampus of Rag1^−/− mice (n = 7/group). H Representative images of Iba-1 immunostaining in the hippocampal DG of Rag1^−/− mice with adoptively transferred Tregs transfecting with the lentivirus Htr7-Flag. Scale bar = 50 μm. I Quantification of Iba-1⁺ cells (n = 7/group). J-M Levels of IL-10 (J), TGF-β (K), TNF-α (L), and IL-1β (M) in the hippocampal DG of Rag1^−/− mice adoptively transferred into Tregs and transfected with the lentivirus Htr7-Flag. (n = 7/group). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns indicates nonsignificant