Fig. 4

5-HT and 5-HT₇ receptor enhanced the immunosuppressive effect of Tregs on CD8⁺ T cells and microglia. A Representative dot plots of flow cytometry analysis of CFSE-positive primary CD8⁺ T cells in cervical lymph nodes. B Quantification of the percentage of target CD8⁺ T cells at different time points. C Representative flow cytometry dot plots showing apoptosis in primary CD8⁺ T cells in cervical lymph nodes. D Quantification of the percentage of apoptotic primary CD8⁺ T cells. E, F mRNA levels of the cytokines perforin (Prf) (E) and granzyme B (GranzB) (F), as measured by qRT-PCR. G Representative Western blot images of Prf and GranzB. H, I Quantification of the relative expression of the Prf protein (H) and the GranzB protein (I). J-K Mean fluorescence intensity (MFI) of MHC-II⁺ (J) and CD206⁺ cells (K). L-O mRNA levels of IL-1β (L), IL-4 (M), iNOS (N), and TNF-α (O). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns indicates nonsignificant