Fig. 3

Tryptophan-rich diet attenuated LPS-mediated neuroinflammation by activating Htr7⁺ Tregs. A Representative images of CD3, Gr-1, Iba-1, and MBP immunostaining accompanied by SMI32 staining in the hippocampal DG 24 h after LPS treatment. Scale bar = 50 μm. B-D Quantification of CD3⁺ (B), Gr-1⁺ (C), and Iba-1⁺ cells (D) (n = 7/group). E Normalized SMI32/MBP ratio (n = 7/group). F-I Levels of IL-10 (F), TGF-β (G), TNF-α (H), and IL-1β (I) in the hippocampus (n = 7/group). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns indicates nonsignificant