Table 1 Primers used in the qPCR studies
From: IL-6 deficiency accelerates cerebral cryptococcosis and alters glial cell responses
Gene | Annealing temperature (Ā°C) | Primer Sequence (5ā-3ā) |
|---|---|---|
Cap59 | 54 | Forward: GAGTGTCTCCGCAACCCGCA Reverse: TACTTGTGCTGCCTCGCCTG |
Grasp | 54 | Forward: AGTTCTTTACCCTACTAGACAG Reverse: TCTCCTCACATTGTCAGATTC |
GAPDH | 56 | Forward: GCTGCYGCTAACATCATCCC Reverse: YGAAATCAGTRGAGACAACA |