Fig. 1

Schematic of the experiment and blood ethanol concentrations (BECs). (A) CIE exposure paradigm. Created with BioRender.com. (B) and (C) BECs were assessed to ensure that pathophysiologically relevant ethanol levels were obtained at the end of the experiment. The mean BECs were 154.98 ± 10.70 mg/dL, 161 ± 10.19 mg/dL, 223.59 ± 20.15 mg/dL, and 259.73 ± 13.73 mg/dL in the CIE-exposed and BBG-treated–CIE-exposed groups of the pharmacologically P2 × 7R-inhibited cohort (B) and wild-type CIE-exposed, and P2 × 7R^−/− CIE-exposed groups of the P2 × 7R^−/− cohort (C), respectively. When compared with wild-type CIE-exposed group, the P2 × 7R^−/− CIE-exposed group showed significant difference in the BEC. A two-tailed t test was used for the statistical analyses. The values are presented as the mean ± SEM; n = 6–9; *** p < 0.0004 and ns = nonsignificant compared with the CIE-exposed mice