Fig. 2

Effects of mast cell deficiency on choroidal neovascularization. (A) RPE/choroid complex flat mounts illustrating the accumulation of mast cells (green) in the CNV lesion (red). Scale bar: 20 μm. (B) Timeline illustration of the laser-induced CNV model in mice. (C) Representative image of RPE/choroid complex flat mount staining with FITC lectin (green) and rhodamine phalloidin (red) in WT mice, Kit^{W−sh/W−sh} mice, and Kit^{W−sh/W−sh} mice reconstituted with BMDMCs at day 7 following laser burn. Scale bar: 50 μm. (D) Quantification of the CNV lesion size in RPE/choroid complex flat mounts (n = 10–13). Statistical analyses were performed using one-way ANOVA. ***p < 0.001, ****p < 0.0001. (E) Experimental timeline for ketotifen fumarate treatment in laser-induced CNV. (F) Representative image of RPE/choroid complex flat mounts after staining with FITC lectin (green) and rhodamine phalloidin (red) from mice at day 7 after receiving laser burn and treatment with vehicle or ketotifen fumarate (KF) at 25 mg/kg. Scale bar: 50 μm. (G) Quantification of the CNV lesion size in RPE/choroid complex flat mounts. Statistical analyses were performed using unpaired t-tests. ****p < 0.0001. n = 7–9. (H) Representative fundus fluorescein angiography images of mice receiving vehicle or KF 7 days after laser burn. (I) Quantification of vascular leakage. Statistical analyses were performed using Welch’s t-test. *p < 0.05. n = 7