Table 1 Components of basal media used for differentiation and iPSC-derived cultures
From: Differential susceptibility of human motor neurons to infection with Usutu and West Nile virus
Name | Reagents and final concentrations | Manufacturer |
|---|---|---|
iPSC medium | Stemflex medium | Thermofisher Scientific |
100 IU/ml penicillin | Lonza | |
100 μg/ml streptomycin | Lonza | |
1X Revitacell | Thermofisher Scientific | |
Differentiation medium | Advanced DMEM/F12 medium | Thermofisher Scientific |
100 IU/ml penicillin | Lonza | |
100 μg/ml streptomycin | Lonza | |
0.1mM non-essential amino acids | Lonza | |
1x N2 supplement | Thermofisher Scientific | |
10ng/ml Human Recombinant neurotrophic factor (NT3) | Stemcell Technologies | |
10ng/ml brain-derived neurotrophic factor (BDNF) | Peprotech | |
200ng/ml laminin | Corning | |
4 μg/ml Doxycycline (DOX) | Sigma | |
Ngn2 neuron-astrocyte co-culture medium | Neurobasal medium | Thermofisher Scientific |
100 IU/ml penicillin | Lonza | |
100 μg/ml streptomycin | Lonza | |
2mM glutamine | Lonza | |
2% B27 supplement without RA | Thermofisher Scientific | |
10ng/ml Human Recombinant neurotrophic factor (NT3) | Stemcell Technologies | |
10ng/ml brain-derived neurotrophic factor (BDNF) | Peprotech | |
4 μg/ml Doxycycline (DOX) | Sigma | |
sMN basal medium | DMEM: Ham’s F12 | Lonza |
Neurobasal medium | Thermofisher Scientific | |
100 IU/ml penicillin | Lonza | |
100 μg/ml streptomycin | Lonza | |
1 x N2 supplement | Thermofisher Scientific | |
1 x B27 supplement without RA | Thermofisher Scientific | |
1 μl/ml 2-Mercaptoethanol | Thermofisher Scientific | |
0.5μM ascorbic acid | Sigma |