Fig. 1

USUV can productively infect and spread within Ngn2 neural co-cultures but not motor neurons. A. Growth kinetics of USUV and WNV on iPSC-derived Ngn2 neural co-cultures infected at MOI 1 and 0.01. 3 replicates per condition, per experiment. n = 3. ** p = 0.0018. *** p = 0.0004. **** p < 0.0001. B. Growth kinetics of USUV and WNV on iPSC-derived motor neuron cultures infected at MOI 1 (n = 3) and 0.01 (n = 2). 3 replicates per condition, per experiment. Mean with SD. **** p < 0.0001. ns = non-significant. Horizontal blue line = USUV MOI 1 0hpi vs. USUV MOI 1 72hpi. Horizontal red line = WNV MOI 1 0hpi vs. WNV MOI 1 72hpi. Vertical black lines = USUV MOI 1 vs. WNV MOI 1. Data displayed has been log-transformed (Y = log[Y]). 2-way ANOVA with multiple comparison carried out on log-transformed data C. IF staining of viral envelope protein showing the distribution of USUV- and WNV-infected cells within iPSC-derived Ngn2 neural co-cultures and motor neuron cultures at 3 and 7 dpi. DAPI is shown in blue. Flaviviral envelope protein is shown in green. Scale bar represents 100 μm. Representative images of 3 experiments. An MOI of 1 was used for infection