Fig. 5

Gas6 Supplementation increased BBB integrity in vivo. Recombinant AAV-gas6 (rAAV-gas6) or rAAV-GFP was constructed and intracerebroventricularly injected into the mouse brains. Four weeks after rAAV-gas6 or rAAV-GFP injection, the mice were infected with JEV via the foot pad (10⁷ PFU). (GFP: rAAV-GFP injected mice; gas6: rAAV-gas6 injected mice) A. Qualitative detection of BBB permeability via EB leakage into brains at 5 dpi. B. Quantitative assay of BBB permeability via NaF extravasation at 5 dpi (Data represent mean ± SD). C, D. Viral copies in the brains of each group at 3 and 5 dpi were tested using qPCR. Data were normalised to β-actin and the relative change of viral copies was calculated based on the viral level of GFP-d3-1 (C) or GFP-d5-1 (D). Data are shown as mean ± SD