Fig. 4

GAS6 increased TJ protein expression and BBB integrity in vitro. A. Proliferation activity of bEnd.3 cells treated with GAS6 or serum free culture medium. B. bEnd.3 (cultured with media or media containing rGAS6) were treated with a homogenate supernatant from JEV- infected mouse brains. Expression of TJ proteins, including claudin5 and occluding, at 24 and 48 hpi. C. Schematic diagram of the in vitro BBB model constructed using bEnd.3, C8-dia and neuro2a cells in a Transwell system. D. In BBB models, neuro2a cells were infected with JEV, bEnd.3 cells were treated with media alone or media containing rGAS6. TEER was detected in all groups. The TEER in rGAS6 treated bEnd.3 cells was higher than that in the other groups