Fig. 2

AIBP deficiency exacerbates TLR4 inflammarafts and oxidative stress in APP/PS1 mice. (A-C) Flow cytometry analysis of CD11b⁺ CD45^low microglia cells in single cell suspensions isolated from age-matched 6-months old female mice (n = 6 per group). (A) TLR4 dimerization, (B) TLR4 expression, and (C) lipid raft content (CTxB). (D and E) Female mouse brain tissues (WT n = 5, APP/PS1 n = 5, and Apoa1bp^−/− APP/PS1 n = 8) were stained with the superoxide indicator dihydroethidium (DHE, 10 µM; red). Representative images in the dentate gyrus (DG) and cortex. Scale bar, 100 μm. Mean ± SEM. One-way ANOVA with Tukey’s multiple comparisons test