Fig. 4

CB2 immunoreactivity in parenchymal microglia of the FC of rats in control conditions (P-CMS-), after periodontitis induction (P + CMS-), after chronic mild stress exposure (P-CMS+), and after both protocols combined (P + CMS+). Immunofluorescence of Iba1 (red), CB2 (green) and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) signals in nuclei (blue) in representative images of 30 mm-thick sections of rat brain FC from the P-CMS- (A), P + CMS- (B), P-CMS+ (C), and P + CMS+ (D) groups was performed. Arrowheads indicate representative cells. Quantitative analysis of CB2 expression in Iba1 + cells (E). The data are presented as the means ± SEMs of 34–36 microglia per group. **p < 0.01. One-way ANOVA was performed following Tukey’s post hoc test. Scale bars = 20 μm