Fig. 1

Increased MHC Class II Expression and α-Syn Phosphorylation in Line 61-PFF Mice. Male Line 61 mice (12–15 weeks old) were unilaterally injected in the striatum with human α-Syn monomer (10 µg) or hPFF (PFF) (10 µg). Four weeks later, those injected with human α-Syn monomer were perfused for immunofluorescence. Two weeks later, those injected with hPFF, were treated with VH (DMSO) or AZD1480 (25 mg/kg/day) for an additional two weeks for a total of four weeks. (A) Immunofluorescence performed in the SNpc detected MHC Class II (magenta), phospho-α-Syn (p-α-Syn) (yellow), or TH (cyan). The abundance of MHC Class II-positive cells or p-α-Syn inclusions was quantified using Fiji. (B) An independent t-test showed significant differences in the number of MHC Class II-positive cells between VH- and AZD1480-treated groups. (C) An independent t-test showed significant differences in the number of p-α-Syn-positive aggregates between groups. Scale bar = 100 μm. *p < 0.05