Fig. 3

Heatmap depicting the expression of 74 neuroinflammatory genes in eight brain regions according to the type of trauma. The values reported were calculated based on the 2^−ΔΔC(t) method and normalized to the geometric mean of three specific, constitutively active, and stable housekeeping genes (B2m, Gapdh, Hprt1) and regional brain biopsy samples from naïve uninjured animals. Blue shading represents a decrease in expression compared to that in the naïve group, red shading represents an increase in expression compared to that in the naïve group, and white shading represents nonsignificant changes (− 2 to 2 fold-change). The color intensity correlates with the magnitude of gene expression relative to that in naïve, uninjured rats