Fig. 1

IRF3 deletion attenuates the proinflammatory effects of acute LPS challenge. A Schematic of the acute LPS challenge model. Sickness behavior was recorded in the open field arena ~ 5 h after i.p. (intraperitoneal) LPS administration and tissue was collected after 6 h. B Quantification of distance traveled and velocity of movement in open field arena shows that IRF3KO mice display attenuated sickness behavior compared to the WT. N = 11–13 for each group. C & D Quantification of ELISA from cortical lysates (1C) and sera (1D) show that proinflammatory cytokines are significantly upregulated in WT mice on LPS challenge but remain significantly reduced in the IRF3KO cortices and serum compared to the WT. N = 11–13 for each group. Two-way ANOVA with Tukey’s multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001