Fig. 7

CB2 mRNA levels positively correlated with inflammatory markers in BV2 cells after IFNγ treatment. Inflammatory genes and CB2 mRNA were quantified by calibrated RT-qPCR in cultured BV2 murine cells following IFNγ 3 h, 8 h or 24 h application (100 ng/mL) and compared with levels quantified in untreated cells (n = 3/condition). A–F Quantification of IL-1β, IL-6, TNFα, COX2, NOS2 and MCP1 transcript levels. G Pro-inflammatory index (PI-I) was calculated from transcript levels of pro-inflammatory genes (A–F). CTRL vs. IFNγ + 8h: p = 0.0132. H Quantification of CB2 mRNA. CTRL vs. IFNγ + 3h: p = 0.0088; CTRL vs. IFNγ + 8h: p = 0.0002; CTRL vs IFNγ + 24h: p = 0.0097. Data are expressed as the mean ± SEM, and presented as relative to untreated BV2 cells. Normal data (IL-1β, IL-6, TNFα, COX2, NOS2, MCP1 and CB2) were analyzed with Tukey’s post-hoc test for multiple comparisons following one-way ANOVA. Non-normal data (PI-I) were analyzed with Dunn’s post-hoc test for multiple comparisons following Kruskal–Wallis test. Asterisks represent control (CTRL) vs respective IFNγ-treated group. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001. I Relationship between of CB2 transcript levels and pro-inflammatory index (simple linear regression, p = 0.0013, R² = 0.6590, y = 0.2545x + 0.5265, n = 12)