Fig. 6

H4K12la activates PD-1 transcription in the injured spinal cord in vivo and microglia in vitro. A The binding density of H4K12la was visualized by deep Tools: the heatmap presents the CUT&Tag tag counted on the H4K12la binding peaks in the spinal cord at Pre and 14 dpi in vivo, ordered by signal strength. B Genome-wide distribution of H4K12la-binding peaks in the spinal cord at Pre and 14 dpi in vivo. C Genome browser tracks of CUT&Tag signal at the PD-1 loci. D ChIP analysis of the indicated promoters was performed using antibodies against H4K12la in the spinal cord at Pre and 14 dpi in vivo. All data are presented as the mean ± SEM, n = 3 mice per group, ****p < 0.0001. E qPCR assays monitoring PD-1 expression in the spinal cord at Pre and 14 dpi in vivo. All data are presented as the mean ± SEM, n = 3 mice per group, **p < 0.01. F ChIP analysis of the indicated promoters was performed using antibodies against H4K12la in primary microglia treated with PBS (control) and lactate in vitro. All data are presented as the mean ± SEM, n = 3 per group, ***p < 0.001. G qPCR assays monitoring expression of the PD-1 in primary microglia treated with PBS (control) and lactate in vitro. All data are presented as the mean ± SEM, n = 3 per group, **p < 0.01