Fig. 8

Hepatic Nfkb deficiency alleviates cognitive performance and improves locomotor activity in R778L mice. (A) Schematic representation of experimental setup. (B) Measurement of total distance, climbing behavior, and time spent in the center of R778L and wild-type (WT) mice injected with siControl or siNfkb, assessed using the open-field test (n = 7–8 per group). (C) Escape latency in mice subjected to the Barnes maze test (n = 7–8 per group). (D) Representative image showing immunofluorescence staining for Ly6G in the livers of R778L and WT mice injected with siControl or siNfkb. (E) Quantification of Ly6G-positive cells per field in livers of R778L and WT mice injected with siControl or siNfkb (n = 6–8 per group). (F) Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) analysis of Tnf mRNA expression in the liver and striatum of R778L and WT mice injected with siControl or siNfkb (n = 7–9 per group). The data are presented as the mean ± SEM and were evaluated using two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the mice treated with vehicle