Fig. 6

sh-AKAP8L and rapamycin mitigated NLRP3 inflammasome and pyroptosis in STZ mice via inhibiting mTORC1 activation. Representative blots (a) and statistical graphs (b) of NLRP3, ASC, Caspase-1, TXNIP, GSDMD-N, and IL-1β. (c) The gene expression of TNF-α, IL-1β, IL-6, CD86, iNOS, IL-4, Arg-1, and TGF-β using Z-score transformation. (d) Representative immunofluorescence images of GSDMD, Iba-1 and DAPI. Scale bar: 20 μm. (e) Representative example binary images of microglia in the hippocampus of mice. (f) Statistical graphs of Sholl analysis results showing the number of intersections per radius around the microglial soma. (g) Quantification of microglial process endpoints per cell. Data are Mean ± SD (n = 6). P-values are from one-way ANOVA with Tukey post hoc multiple comparisons. **p < 0.01 compared to the Control group. ^##p < 0.01 compared to the STZ group