Fig. 5

Sh-AKAP8L and rapamycin alleviated autophagy disorders in the hippocampus of STZ mice by inhibiting mTORC1. (a) Representative blots of p-mTOR, mTOR, p-p70S6K, p70S6K, p-ULK1, ULK1, Beclin-1, p62, LC3-II, LC3-I, and β-actin. Representative immunofluorescence images (b) and statistical graphs (c) of Iba-1 and LC3 colocalization. Scale bar: 20 μm. Representative immunofluorescence images (d) and statistical graphs (e) of Iba-1 and p62 colocalization. Scale bar: 20 μm. (f) Statistical graphs of protein expression for phosphorylation levels of mTOR, p70S6K, and ULK1. (g) Statistical graphs of protein expression for LC3-II/LC3-I, p62, and Beclin-1. Data are Mean ± SD (n = 6). P-values are from one-way ANOVA with Tukey post hoc multiple comparisons. **p < 0.01 compared to the Control group. ^#p < 0.05 and ^##p < 0.01 compared to the STZ group