Fig. 4

Si-AKAP8L and rapamycin alleviated high glucose-induced activation of NLRP3 inflammasomes and pyroptosis in microglia. (a) Representative blots and statistical analysis of NLRP3, ASC, Caspase-1 p20, TXNIP, GSDMD-N, and IL-1β in response to HG treatment. Representative immunofluorescence images (b) and quantification of NLRP3 (c) and GSDMD (d). Scale bar: 20 μm. (e) ELISA quantification of IL-18 levels. Representative images (g) and statistical graphs (f) of microglia stained with YO-PRO-1 for the analysis of pyroptotic cells. Scale bar: 50 μm. (h) Transmission electron microscopy images of pyroptotic cells, with a primary image at 2 μm and an inset at 1 μm scale. Data are Mean ± SD (n = 5). P-values are from one-way ANOVA with Tukey post hoc multiple comparisons. **p < 0.01 compared to the Control group. ^##p < 0.01 compared to the STZ group