Fig. 1

Proteomic analysis identified AKAP8L as a novel biomarker in high glucose-induced microglia. (a) Schematic overview of the proteomic analysis workflow in high glucose-induced microglia. (b) Volcano plot depicting differentially expressed proteins. (c) KEGG pathway enrichment analysis of differentially expressed proteins. (d) Representative immunofluorescence images of IL-1β with statistical quantification. Scale bar: 20 am. Relative mRNA expression of TNF-α (e) and IL-1β (f). (g) Representative Western blots and statistical analysis of P62 protein levels. Representative confocal microscopy images (h) and statistical graphs (i) of mRFP-GFP-LC3 puncta. Scale bar: 5 μm. (j) Representative immunofluorescence colocalization images and statistical graphs of p62 and AKAP8L. Scale bar: 10 μm. Data are Mean ± SD (n = 3–5). P-values are from unpaired Student’s t-tests. *p < 0.05 and **p < 0.01 compared to the Control group