Fig. 4
From: Role of inflammation in a rat model of radiation retinopathy
Photon irradiation induced GFAP overexpression in the retina. (a) GFAP expression (in red) in Müller cells and microglial cells (white arrows) on cryosections of irradiated eyes after 1 week, 1 month, 6 months and on non-irradiated eyes used as control (Ctrl). Nuclei are stained with Dapi in blue. The scale bar represents 50 μm. GCL means ganglion cells layer; INL, inner nuclear layer; ONL, outer nuclear layer; RPE/Ch, Retinal Pigment Epithelium/Choroid. (b to d) Western blots of irradiated and control retinas after 1 week (b), 1 month (c), 6 months (d) and their quantification. The integrated density of the several GFAP specific bands was measured and normalized over the ponceau red staining. n = 4 (M1) or n = 5 (W1, M6), Mann-Whitney statistical test. *p < 0.05; **p < 0.005