Table 1 Forward and reverse primer sequences
Gene | Forward Primer (5’->3’) | Reverse Primer (5’->3’) |
|---|---|---|
Β-Actin | GCAGGAGTACGATGAGTCCG | ACGCAGCTCAGTAACAGTCC |
18s | ACCAGACTTGCCCTCCAATG | AACGGCTACCACATCCAAGG |
HPRT | AGGCCAGACTTTGTTGGATTTGAA | CAACTTGCGCTCATCTTAGGCTTT |
16 S | GCGGCAGAAATGTCGTTTT | CGCTCGTTGCGGGACTTA |
IL-1β | GAAGTTGACGGACCCCAAAA | GCCTGCCTGAAGCTCTTGTT |
TNF⍺ | GCCTCTTCTCATTCCTGCTTG | CTGATGAGAGGGAGGCCATT |
NLRP3 | GTGGATGGGTTTGCTGGGAT | CCTGCTTCTCACATGTCGTCT |
CXCL9 | CAGAGCCAGACAGGGTGAAA | AGCAGTCCCAAATATCCGCA |
CXCL10 | AAGCTATGTGGAGGTGCGAC | TGAGCTAGGGAGGACAAGGA |
IL-13 | AGACTCCCCTGTGCAACGGCA | GGAGACCGTAGTGGGGGCCTT |
KCNN4 | CCAGTCAGGAGGCCACATAG | AGAAAACACAGGAGCAGGGAT |
Stat1 | CAGAAAAACGCTGGGAACAGA | GGTGGTCTCCAGGTCAATCA |
Stat6 | TCCACGAGCTTCACATTGGG | AGGATGGTAGCTTTGGCGTT |
TGFβ | CTGCTGACCCCCACTGATAC | AGCCCTGTATTCCGTCTCCT |
PTGS2 | AATACTGGAAGCCGAGCACC | AAGAAGTGAAGGGACACCCC |
BDNF | CCTGCATCTGTTGGGGAGAC | TGGTCATCACTCTTCTCACCTG |
IFN𝛾 | CTGGAGGAACTGGCAAAAGG | TTGCTGATGGCCTGATTGTC |