Fig. 2

Effects of CAP and FPI on splenocytes in 5xFAD mice. In (A), there are no significant differences in splenic B cells. In (B), CLIP + B cells are significantly elevated in the spleen of 5xFAD mice compared to WT, but this elevation is not affected by CAP treatment or FPI. In (C), the gating for identifying dendritic cells and macrophages. In (D) and (E), no significant differences were observed for dendritic cells (DCs) or CLIP + DCs. In (E) and (F), although there were no significant differences in splenic macrophages, there was a trend towards an elevation of CLIP + macrophages in the 5xFAD mice that was not affected by CAP or FPI. Examination of CD4+ (H) and CD8+ (I) T cells revealed that CAP treatment significantly reduced CD4 + T cells in 5xFAD mice, whereas the CD8 + T cells exhibited a trend towards an increase in the 5xFAD mice that was not influenced by CAP. Conversely, Ly49 + CD8 cells are significantly decreased in the 5xFAD mice (J). Analysis of memory cell subsets (K-O) revealed that central memory CD4+ (M) and naïve CD4 + cells (N) were reduced in 5xFAD mice. CAP treatment, FPI, and FPI + CAP treatment all increased these populations of cells, suggesting that CAP and FPI could be restoring the ability of T cells to activate in the condition of chronic inflammation induced by the genotypic effects of the 5xFAD mice. Data represented as Mean ± SEM; n = 3–4 per group; *p < 0.05, **p < 0.01