Fig. 5

CD36 knockdown reduces pro-inflammatory polarization in BV2 cells via the Traf5-MAPK axis. (A–C) Validation of stable transfection in BV2 cells by western blot and quantification, n = 3/group. (D–F) Assessment of the Traf5-MAPK signaling pathway by western blot and quantification. (G–I) Western blot analysis of iNOS and ARG1 expression. (J, K) qPCR validation of iNOS and ARG1 expression. (L, M) Immunofluorescence images depicting pro-inflammatory (L) and anti-inflammatory (M) polarization in BV2 cells across different experimental conditions. (N, O) Proportional analysis of polarized BV2 cells relative to the total BV2 population. Pro-inflammatory phenotype/Total (N); Anti-inflammatory phenotype/Total (O). Data represent mean ± SEM from 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001