Fig. 2

CD36 knockout mitigates white matter injury. (A, B) Validation of CD36 knockout efficiency via western blot and associated quantification, n = 3/group. (C) Luxol fast blue staining demonstrating reduced myelin loss in the corpus callosum of CD36 knockout mice at day 7 post-TBI. Scale bar: 50 μm. (D) Transmission electron microscopy images showing demyelination in the corpus callosum at day 7 post-TBI. Scale bar: 1 μm. (E) Axonal diameter distribution. (F) G-ratio analysis reflecting myelin thickness relative to axonal diameter. (G) Dual immunofluorescence staining for dephosphorylated neurofilament protein (SMI-32, red) and myelin basic protein (MBP, green) in the corpus callosum at day 7 post-TBI, with DAPI staining nuclei (blue). Scale bar: 50 μm. (H–J) Quantitative analysis of white matter injury, indicated by changes in MBP (H), SMI-32 (I), and their ratio (J). Data represent mean ± SEM for 4 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001