Fig. 6
Carbohydrate source and quantity alters mouse anxiety behavior without impacting motor function or learning and memory. C57BL/6 mice were randomly assigned to control (CD), high glycemic index (HGD), or ketogenic (KD) diet at 6 weeks of age and put through a battery of behavior tests at 26 weeks. A Accelerated rotarod was used to evaluate motor coordination and learning by recording latency to fall from the rod. B Percent freezing in cued fear conditioning was used as a measure of associative memory. C Novel object recognition assessed spatial learning. Preference index was defined Timenovel/(Timenovel + Timefamiliar). Elevated zero maze evaluated anxiety-like behavior via D percent time spent in open zone, E number of open zone entries, and F total distance traveled. All data are shown as mean ± SEM and n = 6–11 per group. One-way ANOVA followed by a Dunnett’s test was used to assess statistical significance, comparing the HGD or KD to their sex-matched CD, wherein *, **, ***p < 0.05, 0.01, and 0.001, respectively