Fig. 2

Laminin and GFAP positive immunohistochemistry

(A) Quantification of laminin positive area coverage and (B) average vessel perimeter size as assessed using laminin positive immunolabelling. (C) Quantification of GFAP positive area coverage and (D) quantification of percentage of colocalization of GFAP with laminin positive immunolabelling. Data are mean ± SD. Two-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.005. (E) Representative images of laminin (green) and GFAP (red) positive immunofluorescent labellingand colocalization indicating astrocytic “end feet” covering of vessels in the cortical grey matter (cortex) and periventricular white matter (PVWM). Scale bar represents 50 μm