Fig. 7

Ammonia up-regulates astrocytes’ AQP4 expression by activating astrocytes, resulting in astrocytes damage. (a) : Immunofluorescence co-expression of AQP4 and GFAP in astrocytes in each group. (b): AQP4 protein expression in astrocytes was detected by western blot. (c) : AQP4 gene expression in astrocytes was detected by QT-PCR. (d): Astrocyte viability was measured. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001