Fig. 6

Ammonia up-regulates astrocytes AQP4 may be the mechanism of SAE. (a) : The expression of AQP4 and GFAP protein in hippocampal tissue was detected by western blot. (b) : The expression of AQP4 and GFAP gene in hippocampal tissue was detected by QT-PCR. (c) : Hippocampal tissue AQP4 co-expression with GFAP was detected by immunofluorescence. (d) : The changes in the level of metabolites in the hippocampus of mice in each group were detected by 1 H-MRS. (e) : Comparison of glutamine levels in serum and hippocampal tissues of mice in each group. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001