Fig. 5

Enrichment of cytokine signaling pathways in EAU retinas. (A) GO enrichment analysis of the upregulated DEGs between EAU and control retinas, which were identified by bulk RNA-seq analysis. Shown are 15 top enriched GO terms each for molecular function, cellular component and biological process. (B) KEGG enrichment analysis of the upregulated DEGs between EAU and control retinas. (C) Barplot showing the indicated signaling pathways ranked based on their differences in overall information flow within the inferred networks. The top signaling pathways (shown in red) were more enriched in control retinas, whereas the bottom ones (shown in green) were more enriched in EAU retinas. (D) GSEA enrichment analysis of the bulk RNA-seq data identified enriched gene sets associated with the chemokine signaling pathway or cytokine-cytokine receptor interaction. (E-G) Chord diagrams comparing IL1 (E), IL2 (F) and IL6 (G) signaling pathways in control and EAU retinas. Edge color denotes the signaling source. Segments with large arrows represent signaling targets and inner bars represent signaling sources in which the colors indicate signaling targets