Fig. 4

Pathological markers in 3xTg-AD females are increased after injury and associated with cytokines in a region dependent manner. A) Heatmap of Pearson’s correlation coefficient of cortical phospho-tau T181 versus each measured cortical protein (columns) within each injury group and time point (rows); red boxes indicate FDR-adjusted p < 0.05; green and purple dots represent Group 1 and 2 proteins, respectively. Green box shows eight out of nine Group 1 proteins cluster together and show correlation coefficients R > 0.50 with phospho-tau T181. B) Total tau is significantly upregulated in the hippocampus at 24 h post-3xCHI and each successive time point compared to sham-injured animals (p = 0.014, p = 0.0021, p = 0.0021, p = 0.035, p = 0.022 respectively, Wilcox). Phospho-tau (T181) is significantly upregulated in the hippocampus 30 min and 24 h post-5xCHI compared to sham-injured animals (p = 0.0058, p = 0.022 respectively, Wilcox) (mean ± SEM). C) Expression of the gene set “Kegg Alzheimer’s Disease” significantly increases after 1xCHI (p = 0.006, t-test with Bonferroni correction) (mean ± SEM). See individual gene changes in Fig. S10