Fig. 5

Plasmablast rhAbs bind to human primary astrocytes. A Representative images for the 8 rhAbs that bind primary human astrocytes. Green: rhAb staining. Red: GFAP. Blue: DAPI. In the merge panels, yellow indicates co-stain of GFAP with the rhAb. The MFI of astrocytic staining is indicated in parentheses. Scale bar: 20 μm. B Scatterplots of the MFI of human primary astrocytes where the rhAbs with positive binding are binned according to cohort (HC or ATM). The average MFI of each cohort is provided to the left of the x-axis. C Alignment of the antibody heavy chain sequences using Clustal Omega (EMBL-EBI). Only those sequences of rhAbs with positive binding to human primary astrocytes are shown and are binned according to cohort (ATM above and HC below). Green boxes demarcate the 3 motifs shared between astrocyte binding rhAbs. One letter codons that underwent replacement mutation are indicated in red letters. Codon positions which have a fully conserved residue in all sequences are indicated by “*”. Conservation between groups of strongly similar properties are indicated by “:”. Conservation between groups of weakly similar properties are indicated by “.”