Fig. 2

IgG purified from serum samples bind neurons and astrocytes in mouse spinal cord. A, B Representative images from the lumbar spinal cord of WT (A) and EAE (B) mice. Green: IgG staining. Red: GFAP. Blue: MAP2. In the merge panels, yellow indicates co-stain of GFAP with the rhAb. The MFI of astrocytic staining is indicated in parentheses. Scale bar: 20 μm. C, D Scatter plots of the mean fluorescent intensity of neuron and astrocyte binding in WT (C) and EAE (D) tissue using IgG purified from patient and control serum. The average MFI of each cohort is provided below the x-axis