Fig. 1

Assessment of the degree of brain damage and behavioral changes in HI mice. A Flow chart of HI modeling and experimental design. Mice are ligated with the right common carotid artery at P7 postnatal, then hypoxia for 1 h (7% O2), tissue harvest is performed at P8, P14 and P60, and behavioral tests are conducted at P60. B Representative images of TTC-stained coronal brain sections from sham and HI mice 24 h post-HI. The red areas indicate the reaction of TTC reagent with mitochondrial respiratory enzymes in living tissues, while the white areas represent infarcted tissue. Notably, large cerebral infarctions are observed in the HI group. C Mean and standard error of the infarct volume (two-tailed unpaired Student’s t-test, n = 6 mice/group). D Representative trace track of mice in Y-maze test. Start arm(S), Old arm(O), New arm(N). E Time to explore within the new arms (two-tailed unpaired Student’s t-test, n = 8 mice/group). F Representative trace track of mice in the novel object recognition test. G Discrimination index (two-tailed unpaired Student’s t-test, n = 8 mice/group). H, I Total distance and center exploration time in the open field experiment (two-tailed unpaired Student’s t-test, n = 8 mice/group). J Immobile time in the forced swimming (two-tailed unpaired Student’s t-test, n = 8 mice/group). K Time in the open arm of the elevated O-maze (two-tailed unpaired Student’s t-test, n = 8 mice/group)