Fig. 2

IL-9 effects on EAE striatal microgliosis and astrogliosis. A-B) Representative confocal microscopy images of coronal corticostriatal slices of CFA and EAE mice treated with ip injections from 0 dpi, stained with IBA1 (green fluorescence, A) and GFAP (red fluorescence, B) antibodies. Scale bar: 40 µm. Black and white binary images of IBA-1 (A) and GFAP (B) clearly highlight IL-9 effect on microgliosis and astrogliosis, respectively. A’-B’) Both IBA1⁺ cell density and GFAP⁺ cell density are reduced in the striatum of EAE-IL9 mice (N = 3, n = 11) compared to EAE-VHL mice (N = 3, n = 10), reaching values comparable to CFA mice (N = 2, n = 6). One-way ANOVA followed by Tukey’s post hoc, ** p < 0.01, **** p < 0.0001