Fig. 6

The heterogeneity and transcription factor regulatory networks of neutrophils during PHE progression. (A) UMAP of unbiased clustering on the cells for neutrophils from the 9-sample dataset contains five neutrophil clusters. (B) Distribution of neutrophils from different groups (G1, G2, and G3) on a UMAP plot. (C) Proportions of all neutrophil subclusters among three groups. (D) GSVA analysis indicates enriched pathways of each subset of neutrophils. Benjamini and Hochberg (BH) FDR procedure was used for multiple testing of significance. (E) Heatmap of each neutrophil subtype’s inferred regulon activity score (RAS) in cluster levels. (F) The connection specificity index (CSI) matrix highlights the regulon-to-regulon correlation across all neutrophil subtypes from different groups. Hierarchical clustering of regulons identifies four distinct regulon modules. The heatmap shows the regulation activity of each module. (G) Heatmap of the CSI matrix across all neutrophil subtypes. The color key from blue to yellow indicates the activity levels from low to high