Fig. 7
From: Implication of system xc− in neuroinflammation during the onset and maintenance of neuropathic pain
Effect of sulfasalazine on spinal mRNA of astrocytic and microglial markers after PSNL surgery in xCT+/+ mice. Three (left panels) and seven (right panels) days post-surgery, the ipsilateral and contralateral quadrants of the lumbar spinal cord were dissected and used for RT-qPCR analysis. GFAP and Iba-1 were used to evaluate astrocytic (A, B) and microglial (C, D) activation, respectively. Other markers were used to assess microglia activation profile towards pro-inflammatory (NOX-2: panel E & F) and anti-inflammatory (Arg-1: panel G & H) phenotype. For all markers, the mRNA levels were normalized to the mean of 3 housekeeping genes (RPL-19, Ywhaz, HPRT-1) and expressed as a percentage of the respective contralateral mRNA level. Data were obtained from 5 different animals per group and are presented as geometric means with SD. Statistical analyses were performed using a one-way ANOVA model. GFAP glial fibrillary acidic protein; Iba-1 ionized calcium binding adapter protein 1; NOX-2 NADPH oxidase 2; Arg-1 Arginase 1; SAS sulfasalazine